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The collected slides were stained with Hematoxylin and Eosin (H&E) for histological inspection using a LEICA (DFC290 HD system digital camera, Heerbrugg, Switzerland) attached to a light microscope with 10, 20, and 40 objective lenses 49

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Then, the fluorescent probe DCFH-DA was used to detect the ROS content in zebrafish larvae, and fluorescence intensity was statistically analyzed using ImageJ

Here we demonstrate that the latter approach is preferable, because, owing to the relatively small number of sorted samples used in building panels, standard machine learning does not optimize effect size and cell-type specificity, causing the model to overfit and underperform when tested in independent data